A study of glycoproteins in human serum and plasma reference standards HUPO using multilectin affinity chromatography coupled with Rplcmsms 159

Ziping Yang, William S. Hancock, Tori Richmond Chew and Leo Bonilla

7.1 Introduction 159

7.2 Materials and methods 160

7.2.1 Materials 160

7.2.2 Isolating glycoproteins using multilectin affinity columns 161

7.2.3 Analysis of glycoproteins on LC-LCQ MS 161

7.2.4 Analysis of glycoproteins on LC-LTQ MS 162

7.2.5 Protein database search 162

7.3 Results and discussion 162

7.3.1 Protein IDs from the plasma and serum samples 162

7.3.2 Comparison between serum and plasma glycoproteomes 179

7.3.3 Comparison of the glycoproteins present in the samples collected from three ethnic groups 179

7.4 Concluding remarks 182

7.5 References 183

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