Analysis

The microarrays were scanned (ScanArray; Perkin-Elmer Life Sciences) for fluorescence using laser excitation at 543 and 633 nm; GenePix 5.0 (Axon Laboratories) was used to quantify the images. For spots with fluorescence signal surpassing an intensity threshold in both color channels [3], the ratio of background-subtracted median sample-specific fluorescence to background-subtracted median reference-specific channel fluorescence was calculated, and ratios from replicate antibody measurements within the same array were averaged.

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