A Teflon mask was applied to each slide creating 16 individual sample wells with 0.65 cm diameter. Prior to printing, glass slides were cleaned and derivatized with 3-cyanopropyltriethoxysilane. Panels of 25-37 capture antibodies were spotted in quadruplicate into each sample well using a Perkin-Elmer SpotArray Enterprise noncontact arrayer equipped with piezoelectric tips, delivering ~350 pL for each 120 mm antibody spot. Antibodies were applied at a concentration of 0.5 mg/mL at defined positions within each ofthe six production chips. Each well of a slide was printed with a single array type, containing panels of 26, 27, 26, 37, 25, and 28 antibodies, respectively, for chips 1-6. (See Supplemental Tab. 1 for a complete listing of antibodies surveyed.)
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