Human plasma (Caucasian American Sample Set; Lot # BDCA02-Heparin) and serum (Caucasian American Sample Set; Lot # BDCA02-Serum) were obtained from the HUPO Specimen Collection and Handling Committee [20]. The total protein concentration of plasma and serum was estimated using a BCA Protein Assay (Pierce Chemical, Rockford, IL, USA). Trypsin digest was performed with porcine sequencing grade modified trypsin (Promega, Madison, WI, USA). HPLC-grade ACN was obtained from J. T. Baker (Phillipsburg, NJ, USA). All reagents and buffers were prepared with Milli-Q water (Millipore, Bedford, MA, USA).

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