Protein database search

Peptide sequences were identified using SEQUEST algorithm (version C1) incorporated in BioWorks software (version 3.1) (Thermo Electron) and Swiss-Prot human protein database. The database search was limited to only those peptides that would be generated by tryptic cleavage. The SEQUEST results were filtered by Xcorr versus charge state. Xcorr was used for a match with 1.5 for singly charged ions, 2.0 for doubly charged ions, and 2.5 for triply charged ions. We set DCn > 0.085 and Rsp < 5. The protein identification (ID) was made based on the corresponding peptide IDs.

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