Aliquots of the samples were prepared, sealed, and stored under the designated condition for the appropriate length of time. When the incubation period was complete, samples were denatured in a solution containing 9 m urea, 2% CHAPS, in PBS, and stored at —80°C. After all the samples were collected, they were simultaneously thawed and processed in duplicate for analysis using the carbox-ymethyl surface employing optimized SELDI-TOF protocols that are detailed in Rai et al., in this issue . This simultaneous processing effectively removed any bias related to sample processing over multiple runs.
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