Stability studies and SELDI analysis

Aliquots of the samples were prepared, sealed, and stored under the designated condition for the appropriate length of time. When the incubation period was complete, samples were denatured in a solution containing 9 m urea, 2% CHAPS, in PBS, and stored at —80°C. After all the samples were collected, they were simultaneously thawed and processed in duplicate for analysis using the carbox-ymethyl surface employing optimized SELDI-TOF protocols that are detailed in Rai et al., in this issue [6]. This simultaneous processing effectively removed any bias related to sample processing over multiple runs.

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