Biological Basis Of Sperm Sexing

Separation of sperm according to the sex chromosome is based on the DNA content of the sperm. Mammalian semen can be sexed because the X-chromosome-bearing sperm that produce females contain about 4% more DNA than do the Y-chromosome-bearing sperm that produce males. The procedure is 85-95% accurate for the selected sex.[1-3] Sorting of live mammalian sperm according to their DNA content first was developed the USDA Beltsville Agricultural Research Center.[4] This flow cytometric sorting procedure for sperm[5-8] is patented and exclusively licensed worldwide for non-human mammals to XY, Inc., a private company. For the sorting procedure, freshly collected sperm are stained with a DNA-specific bisbenzimidazole dye, Hoechst 33342, for approximately 1 hr prior to sorting.[9] Hoechst 33342-stained sperm fluoresce bright blue when exposed to a laser beam of short wavelength light and the X-bearing sperm are differentiated from the Y-bearing sperm because they fluoresce brighter due to their greater DNA content. The fluorescence of each stained sperm is measured in a stream of fluid as it passes in front of a photomultiplier tube (PMT).[10] The resultant data are integrated using a powerful computer. Only the DNA content of properly oriented sperm can be measured because the flat surface of each sperm head must be properly oriented relative to the PMT.[1,2]

Diabetes Sustenance

Diabetes Sustenance

Get All The Support And Guidance You Need To Be A Success At Dealing With Diabetes The Healthy Way. This Book Is One Of The Most Valuable Resources In The World When It Comes To Learning How Nutritional Supplements Can Control Sugar Levels.

Get My Free Ebook

Post a comment