Expression Of Recombinant Proteins In Media Other Than Milk

Secretion of transgene-encoded proteins in the urine of transgenic animals was demonstrated using recombinant genes under the control of kidney-[9] or bladder-[10] specific regulatory sequences. Expression of transgenes in the kidney or bladder of transgenic animals and subsequent secretion in the urine may provide some advantages over the mammary gland as a bioreactor, as the purification of proteins from urine may be facilitated by lower lipid and protein levels in comparison to milk. Additionally, such animals can be used for production of recombinant proteins over the course of their entire life span.

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