The efficiency of somatic cell nuclear transfer, when measured as development to term as a proportion of oocytes used, has been very low (1 2%). A number of variables influence the ability to reproduce a specific genotype by cloning. These include species, source of recipient ova, cell type of nuclei donor, treatment of donor cells prior to NT, method of artificial oocyte activation, embryo culture, possible loss of somatic imprinting in the nuclei of reconstructed embryos, failure of reprogramming the transplanted nucleus adequately, and techniques employed for NT.
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