Hormonal manipulations of the doe include administration of a progestin for nine to 20 days combined with an injection of eCG either 48 h before, or at progestin withdrawal (see [3] in Fig. 2).[10] During the breeding season, progestin treatments <16 days are combined with PGF2a to ensure luteolysis occurs before or at progestin withdrawal. Availability of progestin sources for synchronizing estrus varies among countries and includes intravaginal sponges containing fluorogestone acetate or medroxyprogesterone acetate, and intravaginal inserts containing progesterone. If a progestin is not available, use of GnRH preceding PGF2a is a reasonable approach in does, such as is done in cattle (see [4] in Fig. 2).

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