There are two primary concerns regarding bioavailability of water-soluble vitamins in animal diets and premixes: 1) stability of crystalline vitamins in vitamin and vitaminmineral premixes as well as in diets and supplements; and 2) utilization efficiency of B-vitamin precursors contained in plant- and animal-source feed ingredients. Readers are referred to other reviews for details concerning factors affecting the stability of crystalline vitamins in diets and premixes. Regarding bioavailability of water-soluble vitamins in feed ingredients, a paucity of research data exists, and few feed ingredients have been evaluated. Feedstuffs contain B-vitamins in precursor, coenzyme, or bound forms, and enzymes are required to release the free form of these vitamins prior to absorption from the gut.

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