Successfully Cloned Species

We have seen that the published data suggest that nuclear transfer in some animals is much less successful than in other species. Part of this difference may reflect the intensity of research, and part may be real and reflect another component critical for the successful reprogramming of the donor nucleus nuclear remodeling. In order for a differentiated nucleus to redirect development in the environment of the egg, its particular constellation of regulatory proteins must be replaced by those of the egg in time for the embryo to be able to use the genome of the donor nucleus to transcribe the genes it needs for normal development. It should be noted that scientists have not

Fig. 1 Schematic of procedures for cloning by nuclear transfer. Nuclear transfer requires a source of recipient eggs. The genetic material is removed from the recipient egg (enucleation) using a glass micropipette. Donor cells are collected, cultured, and transferred next to the cytoplast. The cytoplast and donor cell are then fused together and activated so that development begins. The resulting NT embryo is cultured prior to transfer to a surrogate mother.

Fig. 1 Schematic of procedures for cloning by nuclear transfer. Nuclear transfer requires a source of recipient eggs. The genetic material is removed from the recipient egg (enucleation) using a glass micropipette. Donor cells are collected, cultured, and transferred next to the cytoplast. The cytoplast and donor cell are then fused together and activated so that development begins. The resulting NT embryo is cultured prior to transfer to a surrogate mother.

yet succeeded in making cloned dogs or monkeys by somatic cell NT cloning.

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