Anaerobic Degradation of Neutral Fats and Lipids

Fats and lipids are another group of biopolymers that contribute significantly to the COD in sewage sludge, cattle and swine manures, and wastewater from the food industry, e.g., slaughterhouses or potato chip factories (Winter et al., 1992; Broughton et al., 1998). To provide a maximum surface for hydrolytic cleavage by lipases or phospholipases, solid fats, lipids, or oils must be emulsified. Glycerol and saturated and unsaturated fatty acids (palmitic acid, linolic acid, linolenic acid, stearic acid, etc.) are formed from neutral fats. Lipolysis of phospholipids generates fatty acids, glycerol, alcohols (serine, ethanolamine, choline, inositol), and phosphate. Lipolysis of sphingolipids generates fatty acids and amino alcohols (e.g., sphingosine), and lipolysis of glycolipids generates fatty acids, amino alcohols, and hexoses (glucose, galactose). A scheme for anaerobic degradation of fats is shown in Figure 1.6. Sugar moieties and glycerol can be degraded to methane and CO2 by interaction between fermentative and methanogenic bacteria in low-loaded systems or by cooperation between fermentative, acetogenic, and methanogenic bacteria in high-loaded systems. The long-chain fatty acids are degraded by acetogenic bacteria by p oxidation to acetate and molecular hydrogen. If acetate and molecular hydrogen accumulate, the anaerobic digestion process is inhibited (Hanaki et al., 1981). Odd-numbered fatty acids are degraded to acetate, propionate, and hydrogen, and even-numbered fatty acids to acetate and hydrogen (Bryant, 1979). Only at a very low H2 partial pressure, which can be maintained by hydrogen-utilizing methanogens or sulfate reducers, is p oxidation of at least n-butyrate or propionate exergonic. Methanol, ethanol, and ammonia are formed from choline (Fig. 1.6). After hydrolysis, fermentation, and acetogenesis of the fat components in the methanogenic phase acetate, CO2, and hydrogen are converted to biogas. All subsequent intracellular reactions can be influenced by syntrophic interaction via interspecies hydrogen transfer, except for the extracellular initial lipase reaction.

Fig. 1.6 Anaerobic degradation of fats.

Carbohydrates, proteins, fats, and biogenic oils can also be degraded anaerobical-ly under thermophilic conditions. The overall degradation scheme is the same, but the microorganism populations are different (e.g., Winter and Zellner, 1990). Thermophilic fat degradation is becoming more important in practice, since waste fat from fat separators and fat flotates of the food industry are often cofermented in agricultural biogas plants. Since, for hygienic reasons, the input material must be au-toclaved, a thermophilic process should be used, keeping the fat in a melted, soluble form for more effective metabolism. Biogas plants with cofermentation of waste fat residues are considered waste treatment systems (Chapter 11, this volume) and must be designed to meet the hygienic demands relevant to treatment of the respective waste.

Was this article helpful?

0 0
Atkins Low Carb Diet Recipes

Atkins Low Carb Diet Recipes

The Atkins Diet is here. Dr Atkins is known for his great low carb diets. Excluding, Dr Atkins carb counter and Dr Atkins New Diet Revolution.

Get My Free Ebook


Post a comment