All three studies comparing insecticide treatments with combing were performed in areas where some level of resistance to the insecticide employed was either recognised before commencing the study or else identified as part of the study. As a result, the potential effectiveness of the insecticides was limited. Studies evaluating nit combing as an adjunct to insecticide treatment, used combs that differed considerably in material and construction so it was difficult to attribute efficacy, or lack of it, to either the pediculicide or the comb. An in vitro study (IF Burgess, unpublished) has found that many so-called "nit combs" cannot remove nits from hairs drawn between the teeth.

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