Abbreviations

Chemically defined medium complementary DNA (sequence) cES-cell medium cynomolgus ES (cells) colony forming unit CFU-erythroid CFU-granulocyte 2'3'-cyclic nucleotide 3'-phosphodiesterase DMSO, acetamide and propylene glycol (freezing medium) dibutyryl-cyclic AMP direct current diethyl pyrocarbonate Dil-Ac-LDL Dil-acetylated-low density lipoprotein DMEM Dulbecco's modified Eagle's medium DPX di-n-butyl phthalate xylene (mountant) ECs endothelial cells (Chapter 11) EDTA ethylenediaminetetraacetic...

Animal transplantation models

For the validation of in vitro generated, mES cell-derivatives as being pheno-typically functional and having regenerative capacity, animal disease and transplantation models are essential. These disease models, ideally, should demonstrate similar pathological mechanisms and properties to human disease syndromes and may be either induced experimentally or naturally occurring. Several significant murine i.e. mouse and rat models of pancreatic and liver diseases are now established, and have been...

Differentiation of mES cells into muscle cell lineages

When mES cells are induced to form aggregates in vitro, in the absence of those self-renewal signals normally provided by mitotically inactivated feeder layers or LIF, cell differentiation is initiated see Chapter 5 . If aggregates are allowed to form spontaneously in suspension by seeding a culture onto a non-adhesive substratum typically, a bacteriological-grade Petri dish , the number of cells incorporated into each aggregate is variable. But this number can be controlled through the use of...