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mit easier assembly of finished DNA sequence and require the maintenance of fewer clones, which is particularly important when sequencing large genomes. DNA sequencing, however, is only one application of DNA libraries.

DNA libraries are created by generating a set of DNA fragments of the desired size and then attaching those fragments to the appropriate vector sequence. For genomic DNA, the fragments are normally generated by either enzymatic digestion or simple mechanical shearing of all the DNA of the genome, including noncoding sequences. Fragments are then enzymat-ically attached to the vector sequences, in a reaction known as ligation. The collected fragments, now attached to vector sequences, are then moved into the appropriate host organism for growth and evaluation. Conditions are chosen so that only one fragment enters each organism, which can then be grown up into a colony whose individuals all carry the same fragment.

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