The Procedure

All blotting procedures begin with a standard process called gel elec-trophoresis. During this step, DNA, RNA, or proteins are loaded on to an agarose or acrylamide gel (that functions like a molecular sieve) and are then run through an electric field. Two types of gels are commonly used: agarose gels and acrylamide gels. Agarose gels are based on a mesh-work of agar filaments and are most often used to analyze DNA and RNA. Acrylamide gels are based on a meshwork formed from the chemical acrylamide and used most often to analyze proteins. Gels are loaded with a mixture of many differently sized molecules. When pulled through the

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