Fig. 2. (A) Late anaphase I showing the central spindle pole (arrow). Bar: 10 |im. (Courtesy of J. Puro.) (B) Anaphase II spindles. Bar: 10 |im. (Courtesy of J. Puro.)

3. Glacial acetic acid.

6. Entellan (Merck).

8. Insect Ringer's solution: 130 mM NaCl, 2 mMKCl, 0.8 mMCaCl22H2O, 0.2 mM NaHCO3. Store at 4°C.

10. Modified Carnoy fixative: 80% Ethanol : chloroform : glacial acetic acid, 6 : 3 : 1.

11. Schiff's reagent: Add 1 g Basic fuchsin (Merck, C.I. no. 42510) to 200 mL of boiling distilled water. Shake well and allow to cool to approx 50°C, then filter. Add 0.8 g K2S2O7 (Merck) and 8 mL of 1 N HCl, shake, and allow to stand in the dark over night. On the following day, add 4 spoonfuls of activated charcoal (Merck), shake well, and filter. The filtrate should be colorless. Store protected from light at 4°C.

12. Sorensen's phosphate buffer: Add equal volumes of 67 mM NaH2PO4 stock solution and 67 mM KH2PO4 stock solution to reach pH 6.8.

0 0

Post a comment