1. Siliconized cover slips: Place 18 x 18-mm2 cover slips in cover slip racks (Molecular Probes). Use four containers large enough to hold the cover slips in racks. Pour 95% ethanol into two of these and 1% Aquasil (Pierce) in distilled water into the other two. Dip the rack into the Aquasil, then the ethanol. Repeat using the other two washes. Between washes, tap the base of the holder on a paper towel to remove excess. Multiple racks can be stepped through the washes. Allow to air-dry in a covered container that is slightly propped open to minimize dust. Take care that the cover slips remain as dust-free as possible (see Note 2).
2. MAW fixative: In a 2-mL microfuge tube, mix 800 ||L acetic acid, 800 ||L methanol, 73 |L ddH2O. This remains fresh for only 3 h, after which it should be remade.
3. Drosophila Ringer's solution: 182 mM KCl, 46 mM NaCl, 3 mM CaCl2, 10 mM Tris-HCl. Adjust to pH 7.2 with HCl and autoclave.
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