Equipment

1. Small Petri dish for dissections (Corning Glass Works, Corning, NY).

2. Fine-tipped watchmaker's forceps (Sigma-Aldrich, style no. 5)

3. Tungsten needles. Tungsten wire can be purchased from Agar Scientific (www.agarscientific.com) and the needle holder can be obtained from Fisher Scientific. The tungsten needles can be sharpened and shaped by electrolysis in either 10% KOH or 10% NaOH with 2-10 V dc. The NaOH and KOH solutions are harmful and toxic, wear gloves and safety goggles. Use only in a chemical fume hood. Keep them tightly closed and store in a cool dry place.

4. Short- and long-pulled glass Pasteur pipets (Bilbate Precision Glass Ltd., England). Heat up the middle of the thinner region of a long glass Pasteur pipet using a Bunsen burner. Holding the two ends, quickly pull apart the Pasteur pipet. Break off the very thin end of the long-pulled Pasteur pipets and they are ready for use.

5. Scalpel blades (Sigma-Aldrich, stainless steel).

6. Grape juice plates for egg collecting.

7. Microscope slides and 24 x 24-mm2 cover slips.

8. Egg basket and painting brush for embryo handling.

9. Rotating wheel.

10. 3MM Whatmann paper. Cut into pieces that correspond to the size of the microscope slide.

11. Stereo microscope for dissection.

12. Laser Scanning Confocal microscope.

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