Larvae

1. Once you get really skilled you will be able to use nearly anything, but give yourself every advantage at the beginning. Start a fresh culture on the most nutritious food available to you, seeded with yeast; limit larval competition by using only one to three female parents per vial (discard or transfer them to fresh food after 3-5 d); be ready to begin work as soon as the first larvae crawl up out of the food (see Note 4).

2. Outcross the aberration stock to wild type (see Note 5). If the aberration stock already has a balancer with a larval marker, fine, but I do not recommend bothering to rebalance a stock to introduce one: once you have the hang of making the preparations it is easy to make enough of them to be 95% confident of having at least one nonbalancer (i.e., 5) in only a few minutes, and if this part has not become routine yet then you need the practice! Indeed, some aberrations (usually deficiencies) delay development. If you have not gotten a nonbalancer preparation out of say the first 20 larvae up, stop work and try again the next day on the delayed, crawled-up larvae (see Note 6).

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