Mitotic Characterization

1. No. 5 dissection forceps.

2. Dissection dish.

3. Phosphate-buffered saline (PBS), pH 7.0.

4. 0.5% Sodium citrate solution, pH 7.0.

6. Glass microscope slides.

7. 22-mm2 siliconized glass cover slips.

9. Whatman filter paper.

11. Razor blade.

12. 0.5 |g/mL 4',6-Diamidino-2-phenylindole (DAPI) in PBS.

13. Coplin staining jars.

14. Glycerol mounting medium.

15. Compound fluorescence microscope, equipped with 100x objective lens and a filter set for DAPI.

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