Doing the job at hand requires careful attention to details of tissue preparation, fixation history, staining, and the stoichiometry of the Feulgen-DNA dye complex, as well as well as determining optimal acid hydrolysis conditions and selecting several appropriate reference standards to validate staining and measuring techniques.

Care in setting up, calibrating, and using a microdensitometer are very important, as is the need to reduce flare, nonspecific light loss resulting from scatter, and errors resulting from inhomogeneous distribution of stained chro-matin. Relevant particulars about Feulgen staining and the use of a scanning and integrating microdensitometer are described next.

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