1. Collect the emerged adult flies, both mwh +/+ flr3 and mwh +/TM3, BdS genotypes, from both the ST, and HB crosses and store in 70% ethanol. TM3 heterozygotes are identified by the BdS serration on the wing extremity.
2. Rinse the flies in water and then transfer them into a drop of Faure's solution on a slide.
3. Detach the wings from the body.
4. With tweezers, line up on a clean slide 10 female and 10 male wings per slide, ensuring that they are spread out.
5. Keep the wings in a dust-free environment (e.g., in a Petri dish) for at least 24 h, or for 1 h on a hot plate (60°C), because they need to be firmly glued to the slide.
6. Put a droplet of Faure's solution on a cover slip, and with the drop hanging, lower it on top of the wings.
7. Place several metal cubes on top of the cover slip for at least 24 h at room temperature, or for 1 h on a hot plate (60°C), while the preparation dries and hardens.
8. Seal the cover slip with nail polish to obtain a permanent preparation.
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