TUNEL Materials

2. Heptane.

4. Proteinase K (Fisher) stock solution, 20 mg/mL in distilled water (dH2O), stored frozen in 10-|L aliquots.

5. Bovine serum albumin (BSA) (Fisher).

6. Normal goat serum (Gibco-BRL).

7. pH 9 Buffer: 0.1 M Tris-HCl, pH 9.5, 50 mM MgCl2, 0.1% Tween-20.

9. Methanol.

11. ApopTag reagents (Serologicals Corp., Norcross, GA): Equilibration buffer (EB), reaction buffer containing nucleotides labeled with digoxigenin (RXB), terminal deoxyribonucleotidyl transferase (TdT), and Stop-wash buffer (SWB).

12. Roche reagents (Indianapolis, IN): Antidigoxigenin antibody complexed to alkaline phosphatase (anti-DIG-AP), nitroblue tetrazolium salt (NBT) and 5-bromo-4-chloro-3-indolyl phosphate toluidinium salt (X-Phos).

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