Gal Staining

1. 1% Glutaraldehyde in PBS.

2. 20% 5-Bromo-4-chloro-3-indolyl-P-D-galactopyranoside (X-gal) in dimethyl-formamide (DMF). Store at -20°C.

3. X-gal buffer: 150 mMNaCl, 7.2 mMNa2HPO4, 2.8 mMNaH2PO4, 1 mMMgCl2. Keep as 5X stock.

4. Staining solution: X-gal buffer containing 5 mM K4Fe(CN)6, 5 mM K3Fe(CN)6. Store 50 mM stock solutions in the dark.

6. Microscope slides and cover slips for mounting.

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