Conclusions

What is needed to further fine tune the in situ analysis of exopolymeric substances of microbial communities? Primarily, we require a more detailed characterization of the probes and a greater understanding of the nature of their affinities for targets such as protein, lipid, nucleic acids, and carbohydrates. Further studies of their behavior in complex microbial communities must also be carried out in order to establish the "ground truth" for imaging based studies. This has been carried out in part through the correlative application of STXM and 1P-LSM imaging25 which indicated considerable agreement between the probe free and the probe dependent mapping of major biopolymers in biofilm systems. However, new probes specific for major biopolymers will have to continue to be developed and evaluated in complex systems. These highly specific probes will be critical for the assessment of floc properties as well as for structural examination of these microbial communities.

Good Carb Diet

Good Carb Diet

WHAT IT IS A three-phase plan that has been likened to the low-carbohydrate Atkins program because during the first two weeks, South Beach eliminates most carbs, including bread, pasta, potatoes, fruit and most dairy products. In PHASE 2, healthy carbs, including most fruits, whole grains and dairy products are gradually reintroduced, but processed carbs such as bagels, cookies, cornflakes, regular pasta and rice cakes remain on the list of foods to avoid or eat rarely.

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