Contents

6.1 Introduction 122

6.2 Methodology 123

6.2.1 Handling Flocs for Microscopic Examination 123

6.2.2 Epifluorescence Microscopy 124

6.2.3.1 CLSM Limitations 125

6.2.3.2 2P-LSM Limitations 125

6.2.4 Synchrotron Radiation (Soft x-ray Imaging) 126

6.2.4.1 STXM Limitations 127

6.3 Targets and Probes 127

6.3.1 Polysaccharides 127

6.3.1.1 General Probes 127

6.3.1.2 Lectins 128

6.3.1.3 Antibodies 131

6.3.2 Proteins-Lipids 131

6.3.3 Nucleic Acids 131

6.3.4 Charge/Hydrophobicity 132

6.3.5 Permeability 133

6.4 Examination of EPS Bound and Associated Molecules 133

6.5 Digital Image Analyses 134

6.5.1 Quantitative In Situ Lectin Analyses 135

6.6 Deconvolution 136

6.7 3D rendering 136

6.8 Conclusions 137

Acknowledgments 137

References 137

©2005 by CRC Press 121

0 0

Post a comment