Rendering

The use of visualization techniques such as confocal, 2P-LSM, or synchrotron based imaging necessitates the presentation of the data in the most meaningful form. In general, this has meant the use of a range of color and three-dimensional presentation

FIGURE 6.11 (Color Figure 6.11 appears following page 236.) 3D rendering of Elbe river floc showing the individual channels (A) staining with the lectin Triticum vulgaris-TRITC; (B) nucleic acid staining with SYTO9; (C) an autofluorescence signal for algae; (D) reflectance of particulate colloidal materials; (E) merged 3D image; and (F) oblique view of the 3D rendering of the four image stack.

FIGURE 6.11 (Color Figure 6.11 appears following page 236.) 3D rendering of Elbe river floc showing the individual channels (A) staining with the lectin Triticum vulgaris-TRITC; (B) nucleic acid staining with SYTO9; (C) an autofluorescence signal for algae; (D) reflectance of particulate colloidal materials; (E) merged 3D image; and (F) oblique view of the 3D rendering of the four image stack.

formats including: simulated fluorescence, stereo pairs, red-green anaglyph projections, and two or three color stereo pairs. These approaches allowing the presentation of multichannel information sets providing a synthesis of large data sets for the readers examination. Additional approaches involve the application of 3D rendering of these data sets. Figure 6.11 provides an example of a series of images of an Elbe river floc showing: four images, the reflection image of particulate matter in the floc, autofluorescence showing the presence of autotrophic algae and cyanobacteria, staining with the nucleic acid stain Syto 9 showing all bacteria, and staining with a lectin Triticum vulgaris-TRITC revealing the exopolymer matrix of the floc as single channel maximum intensity projection (MIP) and then combined as MIP and a rendering image all of the same floc. Finally, images are often adjusted in terms of color balance for publication using programs such as Photoshop (Adobe Systems Inc., San Jose, California)

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