Holger Daims Contents

15.1 Introduction 317

15.2 Molecular Methods for Analyzing Uncultured Bacteria 318

15.2.1 Phylogenetic Analysis of 16S Ribosomal RNA Gene Sequences . 318

15.2.2 Fluorescence In Situ Hybridization 319

15.2.3 Quantification and 3D Visualization of Microorganisms by Confocal Laser Scanning Microscopy and Digital Image

Analysis 321

15.2.4 Methods to Determine Ecological Functions of Uncultured Bacteria 324

15.3 Discovery and Investigation of Nitrospira-Like Bacteria in Wastewater Treatment Plants 328

15.4 Outlook 331

References 332

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