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Active movement due to signaling or chemotaxis103 Release of proteins involved in physiological protection/detoxification mechanisms leading to deflocculation' Cell lysis leading to biomass reduction35; lysis due to cell membrane damage under phenolic shocks83 Anaerobically mediated reduced hydrophobicity104 Microbial death from a temperature downshift from 50° C to 9.5°C72

Lack of terminal electron acceptors: oxygen under anaerobic conditions; nitrate under anoxic conditions84 Increased feed toxicity to microorganisms due to a temperature downshift from 50°C to 9.5°C72

Decrease in surface hydrophobicity and change in physico-chemical properties,105 and decrease in hydrophobic interactions.77 Decreased sludge hydrophobicity, as measured under phenolic shocks,83 probably due to the effect of lysed microbial products and the amphiphatic properties of phenol Contraction and expansion of adhesive polymers87 Solubilization of EPS, such as of bound lipids by specific solvents (methanol, n-butanol) in yeast aggregates106 Reduced solution ionic strength in sludge whose structure is dependent in double-layer electrostatic interactions or ionic bridging5

Ion exchange of Ca2+ by K+ and Mg2+ released by polyphosphate accumulating bacteria under anaerobic storage Decreased sludge adsorption capacity of contaminants due to

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