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FIGURE 6.9 CLSM images showing the differential penetration of microcolonies with (A) binding and retention of 100 nm sulfate modified beads on the outside; (B) penetration of the 20 nm aldehyde-sulfate modified beads to the inside of the colony; and (C) the overlay of the two channels showing localization of the two probes.

FIGURE 6.10 CLSM micrographs illustrating (A) the binding of Phaseolus vulgaris-TRITC lectin; the development of ELF97 (Molecular Probes, Eugene, OR) phosphatase activity reporting fluorescence (B); and (C) Arachis hypogaea-CY5 lectin binding pattern; and (D) the combination of the three signals shows the presence of both cellular and EPS localized phosphatase activity.

FIGURE 6.10 CLSM micrographs illustrating (A) the binding of Phaseolus vulgaris-TRITC lectin; the development of ELF97 (Molecular Probes, Eugene, OR) phosphatase activity reporting fluorescence (B); and (C) Arachis hypogaea-CY5 lectin binding pattern; and (D) the combination of the three signals shows the presence of both cellular and EPS localized phosphatase activity.

Newport Green may be used to detect the presence of metals in biofilms and within the EPS matrix.

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