Fig. 4. Overlay of ionomycin and thapsigargin responses of Jurkat cells loaded with 0.5 |iMRhod-2. The difference in the responses is a measure of internal stores of calcium released that flux to the mitochondria other than from the ER.

similar to that seen with Indo-1. Analyses can be simultaneously combined with immunofluorescent detection of phycoerythrin-labeled antibodies to enable [Ca2+]i measurement within cell subsets (9). The method below is a simple procedure that uses Fluo-3 alone but has been shown to be effective (10,11).

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