In the past, determining the absolute DNA content of cells has proven to be useful in the prognosis of hematological malignancy. A DNA index (DI) is established to assess abnormal DNA content. Cells are stained with propidium iodide (PI), and the DI is an expression of the ratio of DNA content of Gq/Gj cells in the abnormal population to that of a normal population. It is possible to identify the abnormal peak by comparing its position with nucleated chicken
Fig. 2. Cell cycle analysis. (A) Cell cycle distribution of cells stained with the DNA dye propidium iodide (top). Left column is of control cells and shows a normal cell cycle distribution, center column is of cells treated with the mitotic inhibitor nocoda-zole and shows cells accumulating in mitosis, and right column is of cells treated with a DNA damaging agent and shows cells accumulating in G2. From these DNA profiles, it is difficult to distinguish between mitotic arrest and G2 arrest. The bottom panel of histograms shows the usefulness of staining for a mitotic protein (histone H3 serine 10 phosphorylation) in making the distinction possible. (B) Propidium iodide staining and staining for histone H3 serine 10 phosphorylation can be combined on a dot plot that shows positive antigen staining of mitotic cells.
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