Reagents and Solutions

Point Care Flow Cytometer

Researchers involved in the early work with plant FCM isolated protoplasts with hydrolytic enzyme mixtures, lysed the protoplasts and stained them with a fluoro-chrome, mainly DAPI. Dolezel et al. 1989 give examples of Zea mays Poaceae and Medicago sativa Fabaceae callus and leaf material. Today, the method of preparing a suspension of nuclei for measurement follows the ingeniously simple procedure of Galbraith et al. 1983 . It consists basically of i chopping up the plant material with a sharp...