Metabolic Control For Enhanced Metabolite Production

A number of microbial processes in the production of various food additives involve limiting one or more critical nutrients. The submerged production of citric acid by A. niger involves limiting both iron and phosphate to achieve maximum yields (14). The production and excretion of maximum amounts of glutamic acid by Corynebacterium glutamicum is dependent on cell permeability. Increased permeability can be achieved through biotin deficiency, through oleic acid deficiency in oleic acid auxotrophs, through the addition of saturated fatty acids or penicillin, or by glycerol deficiency in glycerol auxotrophs (15).

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