Capillary Electrophoresis

Capillary electrophoresis (CE) is capable of separating several charged and water-soluble molecules in a single analysis. The method often has similar cleanup requirements as available HPLC methods. An advantage of CE over HPLC is the elimination of organic solvents for the determinative step (125). Using a wide range of methods, CE has been used to separate and quantify aflatoxin, fu-monisins, ochratoxin A and B, zearalenone, moniliformin, and a-cyclopiazonic acid with varying success (125-128). Maragos (127) and Maragos and Greer (125) described procedures for analyzing aflatoxin Bx and fluorescein isothiocyanate-labeled fumonisin in corn using CE with laser-induced fluorescence detection. They reported that the limits of detection for these two toxins obtained by CE were as good as if not better than, the detection limits obtained by HPLC.

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