Characterization of Enzyme Rate Parameters

For a study made for the purpose of establishing basic enzymatic parameters (catalytic rate constant, affinity for substrate, inhibitor binding, etc) the assay must provide appropriate velocity estimates. In general this means the catalytic rate at time zero, that is, when the enzyme and substrate are first combined. Assays involving incubation for a fixed length of time present some difficulties for this purpose. Progress curves, in which the concentration of product is measured at intervals during the incubation, are valid and are not used as often as they might be to determine enzymatic parameters.

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