Chemistry And Analysis

The amino acid composition of a peptide is analyzed after acid- or base-catalyzed hydrolysis by an amino acid analyzer. The covalent structure of a linear peptide (primary structure) is analyzed by a stepwise chemical cleavage from the amino terminus of the peptide using phenyl iso-thiocyanate (Edman degradation) (3). This process is automatically done by a protein sequencer. The stepwise chemical cleavage method from the carboxyl terminus of peptide has also been reported (4). The primary structure of a peptide can be analyzed to some extent after the enzymatic cleavage by aminopeptidase or carboxypeptidase. The primary structure of a peptide can be also analyzed by X-ray crystallography. For the analysis of conformation of the peptides in solution, circular dichloism or nmr are available (5).

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