Induction by Controlling the Culture Conditions

Glutamic acid-producing bacteria such as B. lactofermen-turn require biotin for growth. When the concentration of biotin is controlled in a limited range of 3 to 5 ¡ugfL, the amount of l-glutamic acid accumulated reaches 80 g/L or more if more than 50% sugar is added). If the culture contains more or less than this biotin level, almost no accumulation of l-glutamic acid is observed.

When a small amount of penicillin is added to a culture with an excess amount of biotin, l-glutamic acid production is induced as a result of the inhibition of peptide gly-can synthesis by penicillin in cell walls. When cane molasses is used as a carbon source in cultures in which a sufficient amount of biotin exists, l-glutamic acid is produced by this penicillin method.

Sleeping Sanctuary

Sleeping Sanctuary

Salvation For The Sleep Deprived The Ultimate Guide To Sleeping, Napping, Resting And  Restoring Your Energy. Of the many things that we do just instinctively and do not give much  of a thought to, sleep is probably the most prominent one. Most of us sleep only because we have to. We sleep because we cannot stay awake all 24 hours in the day.

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