Info

Undetermined or nonstandard X

"Weighted mean of relative molecular weight of all residues: 108.7.

'Hydrophobicity coefficients (X) derived from RP-HPLC of peptides, X = (At) (ATAr1 where A is a sequence matrix and b is a mole fraction matrix (5). cBulkiness (Â) used for computation of protein compressibility (6).

Undetermined or nonstandard X

"Weighted mean of relative molecular weight of all residues: 108.7.

'Hydrophobicity coefficients (X) derived from RP-HPLC of peptides, X = (At) (ATAr1 where A is a sequence matrix and b is a mole fraction matrix (5). cBulkiness (Â) used for computation of protein compressibility (6).

eluting the amino acids from the column and quicker elu-tion (less than 30 min) compared to ion exchange chromatography. The fluorometric RP chromatography can accurately quantify 5 pmol of 50-kDa protein, 2-3 times more sensitive than ion exchange/ninhydrin detector systems. Gas-liquid chromatography (GLC) is also being used for amino acid analysis after derivatization of amino acid. The purpose of this derivatization is to make the amino acids volatile by changing them to, for instance, N(0,S)-isobutoxycabonyl methyl esters among many other derivatization techniques. All serum amino acids were measured by gas chromatography (GC) with flame ionization detector using a DB017 capillary column in 9 min. Overall recoveries of amino acids added to serum samples were 88108% (11). Recently, isotope dilution GC/electron capture ionization mass spectrometry was reported for less than 100 fmol amino acids in protein hydrolysates (12).

Progress has been made recently also in the RP/HPLC after precolumn derivatization. Rapid derivatization and direct injection without cleaning is now feasible by using 6-aminoquinolyl-rc-hydroxysuccinimidyl carbamate for derivatization for fluorometric detectors (13). By using phen-ylisothiocyanate for derivatization, a UV detector that is more popular on HPLC instruments can be used with similar detectability as fluorometric detectors (14).

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