Kinetics

The activity of an enzyme is determined by many factors, including enzyme, substrate, and cofactor concentrations; ionic strength; pH; and temperature. For conversion of substrate (S) to product (P) by an enzyme (E) the reaction scheme can be simply represented as

where ES is the enzyme-substrate complex, Ks is the dissociation constant, and Kcat is the turnover number/rate constant for the breakdown of ES.

The initial reaction velocity (V) is then given by the Michaelis-Menton equation

where Km is the substrate concentration at which V equals one-half the maximum velocity (Fmax)- Integration of the equation with respect to time gives

where S and St are the substrate concentrations at zero time and time t, respectively. This equation is particularly useful in industrial situations where a reaction is allowed to proceed to near completion or equilibrium.

Healthy Chemistry For Optimal Health

Healthy Chemistry For Optimal Health

Thousands Have Used Chemicals To Improve Their Medical Condition. This Book Is one Of The Most Valuable Resources In The World When It Comes To Chemicals. Not All Chemicals Are Harmful For Your Body – Find Out Those That Helps To Maintain Your Health.

Get My Free Ebook


Post a comment