Laser Raman Spectroscopy

Raman and infrared techniques are complementary. Raman lines, derived-by a light-scattering effect measured by the emission of radiation from a vibrational exited state, are related to molecular polarizability originating from induced dipole moment, whereas infrared absorption is associated with the permanent dipole moment during molecular vibration. The amide I and II bands are strong in the infrared spectrum, whereas the amide I and III bands are prominent in the Raman spectrum. By analyzing the amide I Raman band directly as a linear combination of amide I bands of proteins whose secondary structures are known from X-ray data, excellent correlation between Raman and X-ray diffraction estimates was obtained for helix, /}-strand, and /?-turn composition (26).

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