Membrane Spread Technique

A "membrane-spread" technique for enumerating Escherichia coli, first described in 1975, used a 90-mm-diameter membrane filter, laid on the surface of an agar plate (6). The sample aliquot was deposited on the membrane filter and spread over the entire surface with a bent glass rod. This 24-hour method, which used bile salts and a 44.5°C incubation temperature for selectivity and an indole reaction as the differential test, was highly specific for E. coli biotype 1. This method, later modified to include a nonselective repair step (7), is recognized as a valid method by the International Commission on Microbiological Specifications for Foods (8).

Berry Boosters

Berry Boosters

Acai, Maqui And Many Other Popular Berries That Will Change Your Life And Health. Berries have been demonstrated to be some of the healthiest foods on the planet. Each month or so it seems fresh research is being brought out and new berries are being exposed and analyzed for their health giving attributes.

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