Membrane Spread Technique

A "membrane-spread" technique for enumerating Escherichia coli, first described in 1975, used a 90-mm-diameter membrane filter, laid on the surface of an agar plate (6). The sample aliquot was deposited on the membrane filter and spread over the entire surface with a bent glass rod. This 24-hour method, which used bile salts and a 44.5°C incubation temperature for selectivity and an indole reaction as the differential test, was highly specific for E. coli biotype 1. This method, later modified to include a nonselective repair step (7), is recognized as a valid method by the International Commission on Microbiological Specifications for Foods (8).

Homemade Pet Food Secrets

Homemade Pet Food Secrets

It is a well known fact that homemade food is always a healthier option for pets when compared to the market packed food. The increasing hazards to the health of the pets have made pet owners stick to containment of commercial pet food. The basic fundamentals of health for human beings are applicable for pets also.

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