Elman and Weiner (18) attempted the clinical application of intravenous casein hydrolyzates in humans in 1939, and the nutritional requirement of amino acids was reported by Rose (19) in 1949. Stimulated by these investigations, various types of protein hydrolyzates containing amino acids were applied in clinical practice throughout the world. But because of the allergic reaction and asymptomatic hyperammonemia that resulted when protein hydrolyzates in the form of administered, crystalline amino acid solutions that did not contain unnecessary components such as peptides were needed. However, it was not until

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