Specific Enzyme Assays

Several assays for enzymes most commonly measured by food scientists will be described. These are only examples

Proteins may be derivatized to fit assay needs. Diazo-tized protein allows measurement of solubilized peptide with a visible-range colorimeter (18). If the amino groups freed during hydrolysis are quantitated using, for example,

Figure 4. Hyperbolic and Hanes plots showing different modes of inhibition.

4 8 12 16 Substrate concentration (a)

Figure 4. Hyperbolic and Hanes plots showing different modes of inhibition.

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