Aluquant Human Dna Quantitation System

The Promega Corporation has developed a human DNA quantitation system that enables sensitive detection of DNA (Mandrekar et al. 2001). The AluQuant™ assay probes Alu repeats that are in high abundance in the human genome (see Chapter 8). Probe-target hybridization initiates a series of enzymatic reactions that end in oxidation of luciferin with production of light. The light intensity is read by a luminometer and is proportional to the amount of DNA present in the sample. Sample quantities are determined by comparison to a standard curve. The AluQuant™ assay possesses a range of 0.1-50 ng for human DNA and can be automated on a robotic liquid handling workstation (Hayn et al. 2004).

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Stammering Its Cause and Its Cure

Stammering Its Cause and Its Cure

This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.

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