Characterizing Cell Lines

STR typing with the same core set of markers and commercial STR kits is being used for several other purposes besides forensic and parentage testing.

Human cell line authentication is now being carried out by the American Type Culture Collection (ATCC) along with other international suppliers of cell lines (Masters et al. 2001). STR typing enables rapid discovery of cross-contamination between cell lines and may serve as a universal reference standard for characterizing human cell lines. This type of analysis has been dubbed 'cell culture foren-sics' (O'Brien 2001). Over the past several years, the ATCC has created a database of over 500 human cell lines that have been run with eight STR loci present in the PowerPlex® 1.2 kit from the Promega Corporation (see Cultures/str.cfm). It is important to note that cell cultures, such as K562 (D.N.A. Box 7.1), may not always have a regular diploid complement of chromosomes and thus may possess tri-allelic patterns and severe peak imbalances due to the presence of additional copies of one allele (Masters et al. 2001).

Was this article helpful?

0 0
Stammering Its Cause and Its Cure

Stammering Its Cause and Its Cure

This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.

Get My Free Ebook

Post a comment