A gel is poured between two glass plates. Any dust particles or other contaminants on the plates can interfere with the collection of fluorescence data by scattering the laser excitation or the fluorescence emission light. Cleaning glass plates is time consuming but essential to obtaining good fluorescent signal with low background. Special low fluorescence plates cost around $150 per pair compared to standard glass plates that are about $50 per pair.
When plates are cleaned, the gel has to be removed since it is bonded to one or both plates. The plates are often soaked to simplify the gel release. Cleaning usually takes about 5-10 minutes for a pair of plates once the gel has been removed.
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This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.